Effect of Various Anesthetics on the Hepatic Microsomal Cytochrome System and the Lipid Contents of Albino Rats

The cytochrome enzyme system of hepatic microsomes is important because of its versatility, being capable of catalysing the hydroxylation as well as other modifications of variety of lipids and foreign compounds, including drugs, pesticides, carcinogens, and anesthetics. In order to elucidate the effect of various anesthetics on hepatic microsomal cytochrom P-450, b₅, and AAF hydroxylation and, on the hepatic lipid contents, inhalation anesthetics, including diethyl ether, halothane, enflurane, and intravenouse anesthetics, including ketamine, thiopental, innovar were administered to albino rats either ones or thrice with the following conclusions: 1. Various anesthetics utilized in the present investigation increase the activities of hepatic microsomal cytochrome P-450 and b₅, with concomitant elevation of AAF hydroxylation in hepatic microsomes. 2. The aove various anesthetics increase the cholesterol and triglyceride contents of liver. 3. The above various anesthetics do not make an influence of the phospholipid content of liver. 4. Variations in the cytochromal enzyme activity of hepatic microsomes have no correlations with variations in the lipid contents of liver by the administration of various anesthetics, while correlations in the contents of hepatic triglyceride and cholesterol have statistically significant correlation with each other by the administration of them.