An Experimental Study for Histopathologic Changes of Rat Urinary Bladder Due to Intravesicle Bacillus Calmette-Guerin Instillation

Bong Suk Shim

Copyright ⓒ 1992. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

The Bacillus Calmette-Guerin(BCG) instillation of urinary bladder as a treatment of superficial bladder tumor can cause inflammatory changes in non-neoplastic area, which occasionally gives difficulty in differentiation from recurrent tumor on follow-up cystoscopy. This study was performed to see the changes of non-neoplastic bladder wall according to the dosage of BCG and time intervals. Low dose(8×10×⁶ CFU/ml) and high dose(12×10×⁶ CFU/ml) as well as normal saline were instilled into bladder of female Wister rats. The histopathological examination was performed on 1, 2, 4 and 8 weeks, respectively.

The following results were obtained;

1) On 2 weeks after intravesical BCG instillation, chronic inflammation with lymphocytes, infiltration was noted on the bladder mucous and muscular layer, hut tuberculous granuloma was not observed. There was not significant difference between Group I and Group II.

2) Chronic inflammatory changes were continuously progressed till 8 weeks, and more severe in Group II than Group I.

3) The denudation of ulcerative epithelial cell was noted in only one case of Group II.

The above results suggest that histopathologic change after BCG instillation on non-neoplastic area was remained upto 8 weeks and need further evaluation for long term effects.