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The Ewha Medical Journal
Ewha Womans University School of Medicine

Ewha Med J 1992; 15(1):49-57

pISSN: 1598-7450

DOI: https://doi.org/10.12771/emj.1992.15.1.49

Original Article

The Study of The Immunoassay by the Monoclonal Antibody: Flow Cytometry for Cell Surface Phenotyping

Ki Sook Hong

Copyright ⓒ 1992. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

With the development of monoclonal antibodies. they have various utilities for in-vitro diagnosis in differentiating tumor cell type, and for therapeutic purposes. I experimented the reaction of many known and new monoclonal antibodies using flow cytometry.

The results are as follows:

1) There were 18 cases of B-lineage Acute lymphoblastic leukemia(ALL)(64%), 8 cases of T-lineage ALL(28%), 1 case of B-myloid ALL(4%) and 1 case of B cell All(4%) in 28 cases of ALL.

2) The reactions with anti-CD9, CD10, CD19, CD24, CD34, CD72 and HLA-DR were positive in most cases of non-T-All, and the reaction with ani-CD13, CD33, CD40 were relatively positive in a few cases of non-T-ALL.

3) The reaction with anti-CD24, CD19, CD20, CD72 and HLA-DR was strong positive in 1 case of Hairy cell leukemia(HCL).

4) There were 17 cases of B-Chronic lymphoytic leukemia(CLL)(71%), and 4 cases of T-CLL(17%) in 24 cases of CLL.

5) There were 8 cases of B-non Hodgkin's malignant lymphoma(NHL)(57%), and 4 cases of T-NHL(29%) in cases of MHL.

6) There were positive reaction with anti-CD2 in normal bone marrow, fetal bone marrow with anti-CD19, tonsil with anti-CD4, anti-CD19, and thymus with anti-CD2.

Immunophenotype with panel of monoclonal antibodies using flow cytometry is very helpful to take diagnosis and treatment for hematologic malignancies.