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The Ewha Medical Journal
Ewha Womans University School of Medicine

Ewha Med J 1995; 18(4):367-374

pISSN: 1598-7450

DOI: https://doi.org/10.12771/emj.1995.18.4.367

Original Article

Simultaneous Detection of Intracytoplasmic CD3ε and Surface CD4 and CD8 Expression by Murine Thymocyte by Flow Cytometry

Young Joo Cho

Copyright ⓒ 1995. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

T-Iymphocytes regulate the erpression of a number of surface antigens such as CD4, CD8 and T cell receptor/CD3 complex, during their intrathymir development. The appearance of surface CD3 is heralded by cytoplasmic CD3 expression in T cell precursors. Thus early T cell progenitors may be distinguished by the presence of cytoplasmic CD3 in the absence of surface CD3. In this study, 1 described a technique for the simultaneous detection of intracytoplasmic CD3 and other surface antigens, CD4 and CD8 in the cells permrabilization by NP-40 fo11owing formaldehyde fixation. The technique results in no shift in the light scatter property andno changes in the expression of surface antigens, Tho present technique permitted the detection of cytoplasmic CD3ε and may prove useful in studying the expression of several cytoplasmic and surface antigens during early T cell development.