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The Ewha Medical Journal
Ewha Womans University School of Medicine

Ewha Med J 1979; 2(3):161-164

pISSN: 1598-7450

DOI: https://doi.org/10.12771/emj.1979.2.3.161

Original Article

Influence of Diazepam, Cimetidine and Sulpiride on the Level of Hepatic Microsomal Cytochrome P450 in Rats

B.H. Kim, Y.A. Ham, Y.S. Hong, N.E. Sung
Corresponding author: N.E.Sung. Department of Biochemistry, College of Medicine, Ewha Womans University, Korea.

Copyright ⓒ 1979. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

Cytochrome P-450 is a terminal oxidase of the hepatic microsomal monooxygenase system associated with the oxidative biotransformation of a varity of lipophilic endogenous and exogenous compounds, and generally is assayed by CO-binding spectro-photometry of dithionite-reduced samples. In well fed normal male rats with or without stress condition, the dffect of antiulcer drug; cimetidine, sulpiride and diazepam on the level of microsomal cytochrome P-450 were determined. Only stress condition produced on significant effect in cytochrome P-450 contents. Administration of cimetidine, sulpiride and diazepam in this condition caused a 44.1%~87.5% increment in cytochrome P-450, diazepam produced the most increase. After diazepam treatment of rats, the peak position of cytochrome P-450 shifted to a longer wave length of 452 nm.