Your browser does not support the NLM PubReader view.

Go to this page to see a list of supporting browsers.
The Ewha Medical Journal
Ewha Womans University School of Medicine

Ewha Med J 1979; 2(4):195-199

pISSN: 1598-7450

DOI: https://doi.org/10.12771/emj.1979.2.4.195

Original Article

Effect of Thiamine Deficiency on Drug Hydroxylation and Level of Cytochrome P-450 in the Rats

Bok Hoi Kim, Young Sook Hong, Nak Eung Sung
Corresponding author: Nak Eung Sung. Department of Biochemistry, School of Medicine, Ewha Womans University, Korea.

Copyright ⓒ 1979. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

Our studies are concerned with cytochrome P-450 content and 2-acetylaminofluorene N-and ring-hydroxylation by thiamine deficient rat liver microsomes. Incubation medium contained 100mM HEPES buffer pH 7.8, 2mM NADPH, 100mM KF, 100nM [14C]-AAF and microsomal protein. After 30min, incubation at 37℃, ring and N-hydroxy-9-14C-AAF formations are assayed by radioactivity measurements after paper chromatography separation. The concentration of cytochrome P-450 is elevated in microsomes from rats fed the deficient diet. It appears that the thiamine deficient rat liver is capable of producing effects on the drug hydroxylation enzymes.