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The Ewha Medical Journal
Ewha Womans University School of Medicine

Ewha Med J 2004; 27(1):33-39

pISSN: 1598-7450

DOI: https://doi.org/10.12771/emj.2004.27.1.33

Original Article

Marker-Genes for Dioxin-Induced Hematopoietic Stem Cell Dysfuction Using the Oligonucleotide-Microarray in Mice

Eun Ju Lee, In Hye Jung, Hyung-Lae Kim
Corresponding author: Department of Biochemistry, College of Medicine, Ewha Womans University, Korea.

Copyright ⓒ 2004. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Mar 30, 2004

Abstract

To develop the biomarkers for the immune dysfunction induced by dioxin, 2, 3, 7, 8-TCDD was administered C57BL/6N mice 0.1µg TCDD/??body weight. Colony forming assay showed that the proliferation potential of hemotopoieti progenitor cells in bone marrow was reduced 35-55% more by earlier exposure. The microarray experiments were duplicated, and the candidates were restricted for the genes expressed greater than 121-fold. Resulting candidates were 55 genes. The expression patterns of the whole genes were analyzed by self-organizing maps (SOM). From these results, we selected the stage-specific genes : one genes (SOM c21 : nadh dehydrogenase subunit 5 gene) for gestational 13.5day, 13 genes (c0 : sialophorin ; spn gene, etc) for postnatal 3 week. The above genes are proposed to be a potential use of biomarker for dioxin exposure in the case of immume dysfunctions.

Keywords: Dioxin; Hematopoietic stem cells; DNA microarray; Gene expression