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The Ewha Medical Journal
Ewha Womans University School of Medicine

Ewha Med J 1981; 4(3):83-89

pISSN: 1598-7450

DOI: https://doi.org/10.12771/emj.1981.4.3.83

Original Article

The Effects of Phenobarbital or Cholic Acid on Hepatic Microsomal Cytochrome P450 and Hydroxylation of 2-AAF in Cholestatic Rats

B.H. Kim, Y.S. Hong, N.E. Sung
Corresponding author: N.E.Sung. Department of Biochemistry, College of Medicine, Ewha Womans University, Korea.

Copyright ⓒ 1981. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

In cholestatic rats, effects of phenobarbital or cholic acid on hepatic microsomal cytochrome p-450 and b5 were investigated. The total contents of both cytochrome p-450 and cytochrome b5 were decreased after bile duct ligation and the administration of estradiol. When cholic acid or phenobarbital was adminstrated in cholestatic rats, the decrease of cytochrome p-450 was prevented. The effects of cholic acid or phenobarbital on both ring-hydroxylation and N-hydroxylation of AAF in cholestatic rat hepatic microsomal fraction were studied. N-hydroxylation of AAF in bile duct ligated rat liver microsomes was reduced by 34%, but ring-hydroxylation was increased by 51%. In estradiol administrated rat, both ring-hydroxylation and N-hydroxylation of AAD was increased by 20 to 25%. In cholic acid administration, both ring-hydroxylation and N-hydroxylation was increased by about 10%. N-hydroxylation of AAF in phenobarbital treated rats was reduced more than ring-hydroxylation was reduced compared to the bile duct ligated group. Estradiol treated group which administrated with cholic acid or phenobarbital exhibited inhibited effects of N-hydroxylation.