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The Ewha Medical Journal
Ewha Womans University School of Medicine

Ewha Med J 1984; 7(2):71-78

pISSN: 1598-7450

DOI: https://doi.org/10.12771/emj.1984.7.2.71

Original Article

Reduction in Binding of Aflatoxin B1 to Rat Liver DNA by Vitamin A and E Pretreatment

Young-Sook Hong
Corresponding author: Young-Sook Hong. Department of Biochemistry, College of Medicine, Ewha Womans University, Korea.

Copyright ⓒ 1984. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

We examined pattern of covalent modification of DNA produced in rat liver after exposure to single dose of aflatoxin B1. DNA-bound aflatoxin B1 adducts obtained from rat liver microsomes were hydrolyzed with weak acid to yield 2, 3-dihydro-3-hydroxy-(N7-guanyl) aflatoxin B1 as major product. This adduct was derived from the aflatoxin B1, 2, 3-dioxide and accounted for approximately 80% of the carcinogen-derived radioactivity incorporated into DNA. Acid hydrolysis of the nucleic acid-aflatoxin B1, adducts also yielded 2,3-dihydro-2,3-dihydroxy-aflatoxin B1 and other minor products. Vitamin A and E pretreatment of rats resulted in reduction in the level of hepatic AFB1-DNA adducts to two-third of the control value. Therefore, administration of vitamin A and E to rats chages in AFB1 metabolism probably decreased modification of DNA during this experiment.