An Experimental Study of the Effect of Radiation and Cis-Dichlorodiammineplatinum(II) on the Rat Kidney

The effect of radiation and Cis-dichlorodiammineplatinum(II)(Cis-DDP) in combination was studied in the renal histopathologic changes of the rat. The histologic evaluation was performed 30 days after the single dose of Cis-DDP(2.5mg/kg) and X-ray irradiation(6,8,10 Gy. single dose) and combination of Cis-DDP and X-ray. For the combined treatment a single dose of Cis-DDP was given 30 min before or immediately after radiation(pre-and post-radiation group).

Cis-DDP alone, showed mild necrosis, degeneration, hemorrhage and focal inflammatory cell infiltrations in the renal tubule and mild congestion of the glomerulus. In radiation alone. edema of the glomerulus and tubular degeneration was seen with 10 Gy and 8 Gy, respectively. With combined chemotherapy and radiation, edema of glomeruli and tubular degeneration was seen with 8 and 6 Gy irradiation, respectively in both pre-and post-radiation group. Tubular necrosis and degeneration and interstitial inflammatory cell infiltrations were more marked in pre-radiation group. When the edema of glomerulus was used as an endpoint, the enhancement ratio was 1.25 in normal rat kidney.