Study on Preparation, Quality Control and Clinical Application of Media and Fetal Cord Serum used in Human In Vitro Fertilization Program

In order to investigate the clinical application of media and fetal cord serum in human In Vetro Ferulization program, its preparation and quality control was performed and the results were as follows.

1) When the in vitro growth and developmental to hatching blastocyst of 2-cell stage mouse embyos was observed three times, the average developmental rate was 84.5% in m-KRB media with an addition of 0.3% bovine serum albumin. And m-KRB media was accepted as adequate to experiment.

When the in vitro growth and development to hatching blastocyst of 2-cell stage mouse embyos was observed three times in m-KRB media with an addition of 10% fetal cord blood serum, if the developmental rate was no significance between test media with control, we approved the serum as safe and used it in human in vitro fertilization program.

2) When the in vitro growth and development to hatching blastocyst of 2-cell stage mouse embyos was observed three times in the contol and Ham's F-10 media was accepted as adequate to experiment.

3) When the safe fetal cord serum was used in the human in vitro fertilization program, the average pregnancy rate was 33.3%.

Judging from above results, the strict quality control of media and fetal cord serum should be necessary for obtaining the high pregnancy rate in human in vitro fertilization program However, this kind of quality control system is very complex and time-consuming, so, therefore, the more effective and simple quality control system should be developed in the near future.