Bile Duct Changes in Albino-Rat Experimentally Infected with Clonorchis sinensis

The main objective of the present study is to identify the evidences of mu-cinous cell metaplasia and goblet cell metaplasia of the bile duct epithelial cells in rats. For the purpose, male albinorats(Winstar strain) were infected with 100 Clonorchis metacercariae. On the scheduled day at one month interval from the end of the 1st month after infection up to the 15th month, three rats were sacrificed by general ether anesthesia. The hepatobiliary tissues were extracted, fixed in 10% formalin solution, sectioned serially and stained with haematoxylin and eosin for histopathologic examination. Alcian blue stain was also applied, if necessary. The results obtained are summarized as follows ; 1) At the end of the 1st month after infection, the bile ducts showed marked epithelial cell hyperplasia, adenomatous tissue proliferation, papillary tissue formation, mitotic figures, atypia and stratification of epithelial cells. The periductal and portal spaces demonstrated a mild fibrosis with inflammatory cells consisted of majority of polymorphonuclear leucocytes and eosinophils. 2) From the 2nd month after infection, adenomatous hyperplastic glands demonstrated some focal mucinous cell metaplasia, with subsequently development of goblet cell metaplasia. Most of fibrosis were replaced by collagen fibers. Chronic mononuclear leucocytes were dominating. 3) From the 4th month to the 6th month, all the pictures reached the zeniths in their degrees, and sustained the peaks up to the 10th month or 11th month, then the figures showed gradual regression.