Studies on the Effect of CCl₄, Phenobarbital and Artemisia Messes-schmidiana val viridis on Biliary Excretion in Rat

After animals were treatd with CCl₄, histological changes were shown in the endoplasmic reticulum, and also microsomal activity was decreased and hepatic necrosis, lipid peroxidation in the liver were repoted. Repeated administration of phenobarbital results in the induction of enzyme that metabolize a number of drugs and, Roberts and Plaa reported that bilirubin output was increased significantly after administration of phenobarbital in rats. Artemisia. was used as folk medicine for the treatment of hyperbilirubinemia. Kiku Tanni(1968) claimed that administration of Artemisia. in patient with acute hepatitis, significantly lowered the bilirubin index, S.G.O.T. and S.G.P.T. The purpose of the this report was to study the effect of CCl₄, phenobarbital. Artemisia on biliary excretion. The results obtained are summarized as follows. 1) Volume of the bile juice was decreased in CCl₄ group but increased in phenobarbital and Artemisia. group. 2) Biliary output was slightly decreased in CCl₄ group but increased in phenobarbital group. 3) Cholate output was decreased in CCl₄ group, but phenobarbital and Artemisia group was shown sighificant increase. 4) Cholesterol output was decreased in CCL₄ group, but increased in phenobarbital group. By the above results, it is felt that after treated with phenobarbital and artemisis following liver damage with CCl₄, detoxication process was shown and the liver damage were protected.