Effect of Topical 1% Na-Hyaluronan on Basan Cell Morphogenesis in Corneal Wound Healing

Chung, Jang-Hyun

doi:10.12771/emj.1997.20.1.83

Ewha Med J 1997; 20(1):83-87

pISSN: 1598-7450

DOI: https://doi.org/10.12771/emj.1997.20.1.83

Original Article

Effect of Topical 1% Na-Hyaluronan on Basan Cell Morphogenesis in Corneal Wound Healing

Jang-Hyun Chung

Author Information & Copyright ▼

Department of Ophthalmolgy, Division of Tissue & Cell Biology, Ewha Medical Research Center, College of Medicine, Ewha Womans University, Korea.

Copyright ⓒ 1997. Ewha Womans University School of Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jul 24, 2015

Abstract

The effect of Na-Hyaluronan(Na-HA) on hemidesmosome morphogenesis was evaluated in n-heptanol induced corneal epithelial wounds, Epithelial wounds were produced by applying a 5.5mm round filter paper, soaked in n-heptanol, on the central cornea for 60 seconds. 1% Na-HA in phosphate buffered saline(PBS) or PBS saline were instilled 4 times a day for 3 days. Epithelial healing rates determined during the first two days were not altered by Na-HA. However, the number of hemidesmosome in the central cornea, which was counted in the basement membrane, significantly increased by the treatment with 1% Na-HA, being 10.0±1.1 in the 1% Na-HA treated group and 6.5±2.5 in the control group. The results suggest that topically applied 1% Na-HA may enhance the formation of hemidesmosome in n-heptanol wounded cornea.

Keywords: Na-hyaluronan; Hemidesmosome; N-heptanol; Cornea; Rabbit