This study was carried out to investigate the effect of bovine serum albumin on in vitro growth and development of mouse(C57BL×CBA) one-cell stage embryos in simple and complex media.

One-cell stage mouse embryos were differentiated to two-cell stage at an identical rate regardless of the nature of the media. simple(m-KRB) or complex(Harn's F-10). When bovine serum albumin was added to the media, one-cell stage embryos were also differentiated to two-cell stage at an identical rate in both simple and complex media.

So. therefore, exogenous factors such as the ingredients of media and protein supplementation did not seem to affect the early in vitro growth and development of one-cell stage mouse embryos to two-cell stage.

But, the late in vitro growth and development of one-cell stage mouse embryos from two-cell stage through blastualtion in complex medium were superior to those in simple medium.

And the late in vitro growth and development in bovine serum albumin supplemented simple medium was better than in bovine serum albumin free simple medium, but the late in vitro growth and development in bovine serum albumin supplemented complex medium was identical to those in bovine serum albumin free complex medium.

However, there is no definite conclusion to be made for including or for omitting protein in the preparation of media for the culture of preimplantation embryos. Rather, there may be some specific needs that depend on the species of embryos and on their stage of development, and perhaps on the purity of other ingredients of the media.

This study was carried out to investigate the correlation of ovarian follicular characteristics in vaginal ultrasonography with serum estradiol level in human in vitro fertilization and embryo transfer(IVF & ET) program hyperstimulated by using of pure follicular stimulating hormone (FSH) and human menopausal gonadotropin(hMG).

The results were as follows:

1) Total amount of FSH and hMG administered in the process of hyperstimulation were 300IU and 772±157.5IU respectively. And the number of follicles which were more than 12mm in diameter in vaginal ultrasonography on the day before the aspiration were 6.0±3.7.

2) The greatest number of follicles which were more than 10mm in diameter were 7.4±3.8 on the day after administration of hCG. The serum estradiol level was increased according to the increase in number of follicles of more than 10mm diameter(r=0.9632)

3) The largest diameter of dominant follicles were 19.9±2.3mm which happened on the day after administration of hCG. The serum estradiol level was increased according to the increase in diameter of dominant follicle(r= 0.9791).

4) The total number of oocytes aspirated was 180 from 35 hyperstimulated cycle, which was equivalent to 5.1±3.9per cycle. Of these, the number of mature oocytes was 4.1±3.8per cycle. The ferilization rate of mature oocytes and cleavage rate of Proombryos were 75.6% and 53.7% respectively.

In conclusion, ovarian follicular characteristics in vaginal ultrasonography had strong postive correlation with serum estradiol level in controlled ovarian hyperstimulation process. So. therefore, vaginal ultrasonography would be an important parameter to be able to replace serum estradiol test in vitro fertilization and embryo transfer program.