The present study investgated if constitutive nitric oxide synthase(cNOS), especially neuronal type, is expressed in gastrointestinal epithelial cells of rat. Expression of cNOS was immunohistochemically determined. Some gastric epithelial cells were found to express cNOS. Although less than that by the gastric epithelial cells, cNOS was also found to be expressed by the intestinal epithelial cells. Thus it is possible that constitutive type of nitric oxide synbthase in gastrointestinal epithelial cells may play a role in normal gastrointestinal function.
Experiments were designed to investigate whether there is any correlation between concentration of plasma nitric oxide and pathological severity of acute rejection after lung allograft.
After single lung allograft, dogs were immunosuppressed with triple standard therapy and divided into 2 groups. Group 1(Immunosuppression ; n=4) was maintained on immunosuppression as controls. In group 2(Rejection ; n=15), triple therapy was discontinued to induce acute rejection from postoperative day 5. Plasma concentration of nitric oxide was measured by chemiluminescence method prior to surgery and at postoperative day 9. At postoperative day 9, dogs were sacrificed and rejection was graded pathologically by a working formulation for the standardization of nomenclature in the diagnosis of heart and lung rejection: lung rejection study group.
Plasma nitric oxide level of day 9 was compared to that of prior to surgery in each group. Correlation between plasma nitric oxide level and pathological grade of acute rejection at day 9 in group 2(Rejection) was examined.
In group 2(Rejection), plasma nitric oxide level fo day 9 was elevated significantly, compared to that of prior to surgery(12.28±2.10 vs 6.46 0.57µM/L ; p<0.05).But plasma nitric oxide level of day 9 didn't significantly correlate with the pathological grades of rejection in group 2(Spearman's coefficient
Plasma concentration of nitric oxide was elevated during acute rejection, but didn't correlate with the pathological severity of rejection.
This study was aimed to investigate whether there is any change in palsma nitric oxide during acute rejection of infection after lung allotransplantation.
After lung allotransplantation, dogs were immunosuppressed with standardized triple therapy and divided into 3 groups : in group 1(control: n=4), immunosuppression was maintained; in group 2(n=7), triple therapy discontinued to induce acute rejection at the postoperative day 5; in group 3(n=6), infection was inudced by bronchoscopic inoculation of
During acute rejection period, plasma nitric oxide concentration was found to be elevated significantly at postoperative day 9, compared to day 0(11.52±2.58 vs 6.01±0.88uM/L ; p<0.05). However, plasma nitric oxide concentration wasn't altered by the E. coli-induced infection(14.53±5.19 vs 6.12±0.98uM/L ; p>0.05). Plasma nitric oxide of day 9 weren't different in three groups(p>0.05).
Plasma nitric oxide may be a good marker for acute rejection after allotrans-plantation, but not for infection.
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