A 41-year-old male visited Ewha womans university hospital with the symptom of frequency, dysuria and residual urine sensation. We could find a Mullerian duct cyst by Transrectal ultrasonography(TRUS) and MRI image. Mullerian duct cyst was incised by transutricular seminal vesiculoscopy(TUS). The symptom was decreased significantly after surgical treatment. Presently, We report this case with a review of the Mullerian duct cyst.