The present study was undertaken to determine the effect of estrogen and progesterone on the concentration of estradiol receptor in uterine tissue at the early pregnant rats. The result obtained are as follows : 1) The uterus wet weights of ovariectomized rats were decreased, while the concentration of estradiol receptor(E₂R) was increased, it seems to be well correlated with the low level of estrogen. 2) In the rats treated with estradiol, at 24 hours after treatment, the concentration of E₂R was higher than control-vehicle treatment-groups. 3) In the rats treated with estrogen and progesterone, the concentration of E₂R decreased, whereas estrogen primed rats treated with progesterone, the concentration of E₂R increased relatively. It is, therefore, concluded that the concentration of cytosol E₂R seems to be correlated with the peripheral estrogen and progesterone levels and then cytosol E₂R transfers into nuclear estradiol receptor to stimulate mRNA transcroption for the development of uterine tissue.

The purpose of the present study is to measure concentration of plasma estradiol and progesterone and to determine the concentration of the estradiol receptor in the uterine endometrium at the early pregnant rat.

The results obtained were as followings:

1) Plasma estradiol concentration showed the highest value on day 1 and the lowest on day 2, increasing again on day 3 and 4.

2) Concentration of plasma progesterone increased sharply on day 3, maintaining gradual increase thereafter. Rats ovariectomized on day 2, however maintained lower progesterone levels than those of day 1, throughout the preimplantational period.

3) The concentration of nuclear estradiol receptor seemed to be well correlated with the concentration of plasma estradiol throughout the preimplantation peried. Treatment of estradiol appeared to increase the concentration of estradiol receptor on day 3 and 6 in ovariectamized rats.

4) The differentiation of the uterine endometrial tissue seemed to be closely related to ovarian steroid hormones.

In vivo treatment of theophylline, we have obtained results and summarized them as below. 1) Effects of theophylline on ovulation, and maturation of the oocytes in mouse; (1) The group treated with theophylline at the time of PMS injection, showed marked decrease of the ovulation rate. (2) If theophylline was given to the animals at the time of injection of HCG, the maturality of the oocytes in the Graafian follicles seemed to be inhibited. 2)Effects of the theophylline on the maturality of the oocytes ovulated, and fertilizability of the eggs. (1) The female mice injected with theophylline with PMS remarkably desreased in mating rate. (2) If treatment of theophylline was made with HCG injection, oocyte maturation was inhibited. (3) The production of 2-cell embryo markedly decreased in either group treated with theophylline with PMS or with HCG. 3) Theophylline effect on implantation of the embryos. (1) Pregnancy rate was markedly decreased in both group of control and theophylline treatment.

Citations

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• Cyclic nucleotide phosphodiesterase inhibitors: possible therapeutic drugs for female fertility regulation
  Anumegha Gupta, Ashutosh N. Pandey, Alka Sharma, Meenakshi Tiwari, Pramod K. Yadav, Anil K. Yadav, Ajai K. Pandey, Tulsidas G. Shrivastav, Shail K. Chaube
  European Journal of Pharmacology.2020; 883: 173293.     CrossRef