Abstract

• In order to investigate the effect of bovine serum albumin of in vitro growth and development of F₁ hybrid mouse(C57BL×CBA) two cell stage embryos fertilized in vivo in simple(m-KRB) and complex(Ham's F-10) media, this study was performed and the results as follows are obtained.
  1) When the early preimplantation in vitro grown and development to the stage of 4 cell or more was observed, in simple media the growth and development was suppressed significantly with an addition of bovine serum albumin and in complex media the growth and development was enhanced significantly with an addition of bovine serum albumin.
  When the comparison was made between simple media with bovine serum albumin and complex media with bovine serum albumin, the growth and development in complex media with bovine serum albumin was significantly greater.
  2) When the late perimplantation in vitro growth and development to the stage of blastocyst or hatched blastocyst was observed, in simple media the growth and development was suppressed significantly with an addition of bovine serum albumin and in complex media the growth and development was enhanced significantly eith an addation of bovine serum albumin.
  When the comparison was made between simple media with bovine serum albumin and complex media with bovine serum albumin was significantly greater.
  Judging from this, in preimplantation in vivo growth and development of two cell stage mouse(C57BL×CBA) embryos feritlized in vivo the media with additional nutrients and protein substances that might be mush larger amount than usual would be necessary.
  However, as the preimplantation in vitro growth and development might be variant according to the strains of mouse and different stages of the growth and development every effort should be made to clearify the characteristics of preimplantation in vitro growth and development among various animal species and different development stages.
  So, therefore, human in vitro fertilization and embryo transfer program would be much progressed with an aid of such experimental researches.