Abstract

• The media for assisted fertilization from Medi-Cult is based on a Synthetic Serum Replacement(SSR). The main principle of SSR is a metal iron buffer containing a balanced mixture of iron and trace metals. Recently, Synthetic Serum Supplement(SSS) and Medi-cult are commonly used.
  These synthetic substitutes have some advandages, such as safty from viral contamination and no needs of bioassay.
  To compare the usefulness between the Medi-cult and Ham's F-10 supplemented with fetal cord serum for the culture medium of in vitro fertilization, fertilization rate, cleavage rate and pregnancy rate were studied. It is concluded that Medi-cult was better than Ham's F-10 supplemented with fetal cord serum according to fertilization rate, cleavage rate, pregnancy rate and ongoing pregnancy, but that differences did not have statistical significance.