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A Study of in situ PCR in the Tissue Sections of Borderline Tuberculoid Leprosy without the Bacilli under the Fite Stain

Hae Young Choi
The Ewha Medical Journal 1996;19(2):193-197. Published online: July 24, 2015

Department of Dermatology and Molecular Biology Section of Medical Research Center, College of Medicine, Ewha Womans University, Korea.

Copyright © 1996. Ewha Womans University School of Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • In situ PCR was applied to the paraffin embedded tissue section of two borderline tuberculoid patients which did not show acid fast bacilli on the Fite stain. In situ PCR was performed with direct detection of Digoxigenin incorporated into PCR product. The best condition of the direct in situ PCR was pretreatment with 0.2N HCl for 40 minutes and with 10㎍/ml of proteinase K at 37℃ for 5 minutes and 30 cycles of PCR. The positive signal was observed within the cytoplasm of the most Schwann cells, epithelioid cells and a few endothelial cells.
  • Keywords: in situ PCR; Borderline tuberculoid leprosy

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      A Study of in situ PCR in the Tissue Sections of Borderline Tuberculoid Leprosy without the Bacilli under the Fite Stain
      Ihwa Ŭidae chi. 1996;19(2):193-197.   Published online July 24, 2015
      DOI: https://doi.org/10.12771/emj.1996.19.2.193
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      A Study of in situ PCR in the Tissue Sections of Borderline Tuberculoid Leprosy without the Bacilli under the Fite Stain
      Ihwa Ŭidae chi. 1996;19(2):193-197.   Published online July 24, 2015
      DOI: https://doi.org/10.12771/emj.1996.19.2.193
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      A Study of in situ PCR in the Tissue Sections of Borderline Tuberculoid Leprosy without the Bacilli under the Fite Stain
      A Study of in situ PCR in the Tissue Sections of Borderline Tuberculoid Leprosy without the Bacilli under the Fite Stain
      Ewha Med J : The Ewha Medical Journal

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