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Original Article

Establishment of Three Dimensional In Vitro Laboratory Model in Squamous Cell Carcinoma of the Head and Neck: Spheroid Model and Raft Culture Model

Sung-Wan Byun, Chun Dong Kim
The Ewha Medical Journal 2001;24(1):15-21. Published online: March 31, 2001

Department of Otolaryngology, College of Medicine, Ewha Womans University, Korea.

Copyright © 2001. Ewha Womans University School of Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • Objective
    To establish new in vitro model systems that better reflect in vivo condition, multicellular tumor spheroids(MTS) and raft culture were developed using cell lines of squamous cell carcinoma(SCCHN) of the head and neck in these 3-dimensional systems.
  • Materials and Methods
    Four SCCHN cell lines were used for MTS and raft culture.
  • Results
    All cell lines formed MTS, but only Tu-138 showed a good stratification at the airliquid interface in the raft culture system.
  • Conclusions
    MTS and raft culture system were established successfully from the SCCHN cell lines.
  • Keywords: Multicellular tumor spheroid; Raft culture; Squamous cell carcinoma of the head and neck

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      Establishment of Three Dimensional In Vitro Laboratory Model in Squamous Cell Carcinoma of the Head and Neck: Spheroid Model and Raft Culture Model
      Ihwa Ŭidae chi. 2001;24(1):15-21.   Published online March 31, 2001
      DOI: https://doi.org/10.12771/emj.2001.24.1.15
      Download Citation
      Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.
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      Establishment of Three Dimensional In Vitro Laboratory Model in Squamous Cell Carcinoma of the Head and Neck: Spheroid Model and Raft Culture Model
      Ihwa Ŭidae chi. 2001;24(1):15-21.   Published online March 31, 2001
      DOI: https://doi.org/10.12771/emj.2001.24.1.15
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      Establishment of Three Dimensional In Vitro Laboratory Model in Squamous Cell Carcinoma of the Head and Neck: Spheroid Model and Raft Culture Model
      Establishment of Three Dimensional In Vitro Laboratory Model in Squamous Cell Carcinoma of the Head and Neck: Spheroid Model and Raft Culture Model
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