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Original Article

T Cell Receptor Rearrangement in NKT Cells Differentiated from Cord Blood CD34+ Cells

Yun-Jae Jung, So-Youn Woo
The Ewha Medical Journal 2003;26(2):137-142. Published online: June 30, 2003

Department of Microbiology, College of Medicine, Ewha Womans University, Korea.

Copyright © 2003. Ewha Womans University School of Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • Objectives
    To investigate the time of rearrangement of the TCR gene in the process of NKT cell differentiation from CD34+ human cord blood cells in vitro.
  • Methods
    We isolated the CD34+ human cord blood cells and induced the differentiation of NKT cells by liquid culture including IL-15, FL and SCF for 30 days. In order to detect the time of TCR gene rearrangement in differentiated NKT cell, we performed PCR for TCR-rearrangement excision circles (TRECs) with isolated DNA.
  • Results
    Signal joint TREC first appeared on day 4 or day 8 and continuously existed until day 30. Between day 9 and day 21, cells showed high output of coding joint TRECs after signal joint rearrangement.
  • Conclusion
    In differentiated NKT cells, TCR gene rearrangement started within a week after culture started and mostly occurred in 2 to 3 weeks after culture started.
  • Keywords: NKT cell; CK34+ cells; TCR gene rearrangement

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      T Cell Receptor Rearrangement in NKT Cells Differentiated from Cord Blood CD34+ Cells
      Ihwa Ŭidae chi. 2003;26(2):137-142.   Published online June 30, 2003
      DOI: https://doi.org/10.12771/emj.2003.26.2.137
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      T Cell Receptor Rearrangement in NKT Cells Differentiated from Cord Blood CD34+ Cells
      Ihwa Ŭidae chi. 2003;26(2):137-142.   Published online June 30, 2003
      DOI: https://doi.org/10.12771/emj.2003.26.2.137
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      T Cell Receptor Rearrangement in NKT Cells Differentiated from Cord Blood CD34+ Cells
      T Cell Receptor Rearrangement in NKT Cells Differentiated from Cord Blood CD34+ Cells
      Ewha Med J : The Ewha Medical Journal

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