To establish new in vitro model systems that better reflect in vivo condition, multicellular tumor spheroids(MTS) and raft culture were developed using cell lines of squamous cell carcinoma(SCCHN) of the head and neck in these 3-dimensional systems.

Four SCCHN cell lines were used for MTS and raft culture.

All cell lines formed MTS, but only Tu-138 showed a good stratification at the airliquid interface in the raft culture system.

MTS and raft culture system were established successfully from the SCCHN cell lines.