Maternal ingestion of alcohol produce not only change of drug metabolism but also proliferation of hepatic smooth endoplasmic reticulum and many developmental defects of the central nervous system.

The present investigation examined the effects of maternal consumption of alcohol during pregnancy and/or lactation the activities of electron transfer components, mixed function monooxygenase, UDP-glucuronosyltransferase and lipid peroxidation of neonatal rat liver microsomes. Normal group consisted of neonatal rats whose mothers received standard chow and water. The subject of experimental group were neonatal rats whose mothers were exposed to alcohol during pregnancy and lactation(3 weeks).

The results were obtained as follows :

The activities of the electron transfer system, such as cytochrome P-450, NADPH-cytochrome C reductase were increased in hepatic microsomes of experimental group.

The activities of the mixed function monooxygenase, p-nitroanisole-O-demethylase and the conjugated enzyme, UDP-glucuronosyltransferase were increased in hepatic microsomal experimental group. There was no significant differences between the formation of lipid peroxide of normal and experimental group.

These results suggest that prenatal exposure to alcohol are influenced by disturb of liver microsomal drug metabolism, especially during the fetal period.

Ethanol enhances the activity of microsomal enzyme system and lipid peroxidation. We observed the effect of chronic ethanol administration on cytochrome P-450 level and lipid peroxidation in rat liver microsome. The results were as follows : 1) When rats were administered with ethanol, the level of microsomal cytochrome P-450 was decreased and lipid peroxidation did not change significantly. 2) When vitamin A and E were added incubation medium in each group, lipid per-oxidation was decreased significantly.

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• Effects of Chronic Consumption of Liquor prepared with Gastrodiae rhizoma on Antioxidant Activity in Brain Tissue of Rats
  In-Jeong Yun, Hyun-Joo Kong, Jung-Hyeon Jang, Kyung-Mi Yang
  Journal of the East Asian Society of Dietary Life.2016; 26(6): 531.     CrossRef

We observed the effect of the administration of vitamin A,C and E with β-naphthoflavone and piperonyl butoxide on cytochrome P-450 level and lipid peroxidation in rats liver. The level of hepatic cytochrome P-450 decreased after vitamin A,C and E was administration. In contrast, β-naphthoflavone, when administered with vitamin A,C and E, the increase of cytochrome P-450 was prevented. Lipid peroxidation was decreased after vitamin A, C, and E was administered. Moreover when β-naphthoflavone was administered together with vitamin A, C and E lipid peroxidation was not increased. When piperonyl butoxide was administered together with vitamin A,C and E, both cytochrome P-450 and lipid peroxidation were decreased. These results indicate that vitamin antioxidants can prevent lipid peroxidation by a cytochrome P-450 dependent terminal oxidase system in rat liver microsomes.